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SEC61 Alpha Polyclonal antibody

SEC61 Alpha Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24935-1-AP

Synonyms

Sec61 alpha 2, SEC61A, SEC61A2, SEC61alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of mouse brain using 24935-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of mouse liver using 24935-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HEK-293 cells using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of HEK-293 using 24935-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of U2OS cells using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of U2OS using 24935-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61 Alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    IF Staining of HepG2 using 24935-1-AP

    Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, U2OS cells, mouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24935-1-AP is SEC61 Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC61 Alpha fusion protein Ag20779
Full Name Sec61 alpha 2 subunit (S. cerevisiae)
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC026179
Gene Symbol SEC61A2
Gene ID (NCBI) 55176
RRIDAB_2879807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

Protocols

Product Specific Protocols
WB protocol for SEC61 Alpha antibody 24935-1-APDownload protocol
IF protocol for SEC61 Alpha antibody 24935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
View Article
ratCoIP

iScience

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors

Authors - Angela L. Whittsette
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, but it also recognized a similar band in solo loading buffer.

  • Applications: Western Blot