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  • KD/KO Validated

SENP5-Specific Polyclonal antibody

SENP5-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19529-1-AP

Synonyms

SENP5, SENP5-Specific, Sentrin specific protease 5



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19529-1-AP targets SENP5-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SUMO1/sentrin specific peptidase 5
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberNM_152699
Gene Symbol SENP5
Gene ID (NCBI) 205564
RRIDAB_10643377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.

Protocols

Product Specific Protocols
WB protocol for SENP5-Specific antibody 19529-1-APDownload protocol
IHC protocol for SENP5-Specific antibody 19529-1-APDownload protocol
IP protocol for SENP5-Specific antibody 19529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

EMBO Rep

The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1.

Authors - Emily Waters
humanWB

J Cell Sci

SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.

Authors - Bin Yu
humanWB

J Cell Sci

SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.

Authors - Bin Yu
human,mouseWB

iScience

Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.

Authors - Seiya Yamada
  • KD Validated
mouseWB,IHC,IF

J Comp Neurol

Synaptic localization of the SUMOylation-regulating protease SENP5 in the adult mouse brain.

Authors - Hiroki Akiyama
  • KD Validated
mouseWB

Cell Rep

SENP7 deSUMOylase-governed transcriptional program coordinates sarcomere assembly and is targeted in muscle atrophy

Authors - Mamta Amrute-Nayak