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Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 12 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
19529-1-AP targets SENP5-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | SUMO1/sentrin specific peptidase 5 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | NM_152699 |
| Gene Symbol | SENP5 |
| Gene ID (NCBI) | 205564 |
| RRID | AB_10643377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| IP protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| WB protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 | ||
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
iScience Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.
| ||
Front Biosci (Landmark Ed) SUMO-Specific Peptidase 5 Promotes Oesophageal Squamous Cell Carcinoma Growth through the NF-κB-SLC1A3 Axis
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