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SLC38A4 Polyclonal antibody

SLC38A4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20857-1-AP

Synonyms

Amino acid transporter A3, ATA3, NAT3, PAAT, SLC38A4, SNAT4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC38A4 Polyclonal antibody (20857-1-AP)

    WB analysis using 20857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20857-1-AP (SLC38A4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human kidney using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human kidney using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human liver cancer using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human liver cancer using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human liver cancer using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of human liver cancer using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of mouse liver using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC38A4 Polyclonal antibody (20857-1-AP)

    IHC staining of mouse liver using 20857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, L02 cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20857-1-AP targets SLC38A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC38A4 fusion protein Ag14827
Full Name solute carrier family 38, member 4
Calculated Molecular Weight 547 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC101827
Gene Symbol SLC38A4
Gene ID (NCBI) 55089
RRIDAB_10696186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC38A4 antibody 20857-1-APDownload protocol
IHC protocol for SLC38A4 antibody 20857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
View Article
humanWB,IF

Pathol Res Pract

Assisted reproductive technology causes reduced expression of amino acid transporters in human full-term placentas

Authors - Qingge Jia
View Article