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SNRPD1 Polyclonal antibody

SNRPD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10352-1-AP

Synonyms

HsT2456, Sm D autoantigen, Sm D1, SMD1, snRNP core protein D1, SNRPD, SNRPD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using SNRPD1 Polyclonal antibody (10352-1-AP)

    WB analysis of HEK-293 using 10352-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SNRPD1 Polyclonal antibody (10352-1-AP)

    WB analysis of HL-60 using 10352-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SNRPD1 Polyclonal antibody (10352-1-AP)

    WB analysis of HEK-293 using 10352-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10352-1-AP (SNRPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10352-1-AP targets SNRPD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPD1 fusion protein Ag0364
Full Name small nuclear ribonucleoprotein D1 polypeptide 16kDa
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC001721
Gene Symbol SNRPD1
Gene ID (NCBI) 6632
RRIDAB_2193875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD1 is one Sm components of the small nuclear ribonucleoprotein complexes(snRNPs). It's a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. This antibody detectes 13-16 kDa (SMD1) and 30 kDa (SMD1/SMD2 dimer form; PMID:15525645).

Protocols

Product Specific Protocols
WB protocol for SNRPD1 antibody 10352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
View Article
mouseIHC,WB

Pharmacol Res

Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses.

Authors - Jing Wang
View Article
mouseWB

J Proteome Res

Signaling in insulin-secreting MIN6 pseudoislets and monolayer cells.

Authors - Chowdhury Azazul Islam AI
View Article