SORLA Polyclonal antibody

SORLA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22592-1-AP

Synonyms

C11orf32, LR11, LRP9, SORL1, SORLA, SorLA 1, Sortilin related receptor



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22592-1-AP targets SORLA in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SORLA fusion protein Ag18445
Full Name sortilin-related receptor, L(DLR class) A repeats-containing
Calculated Molecular Weight 2214 aa, 248 kDa
Observed Molecular Weight 300 kDa
GenBank Accession NumberBC137171
Gene Symbol SORL1
Gene ID (NCBI) 6653
RRIDAB_2879131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SorLA also known as SorL1 or LR11, is a multiligand receptor belonging to the LDL receptor superfamily. It is also a member of vacuolar protein sorting-10 (Vps10) family and is enriched in the brain. SorLA acts as an sorting receptor for APP and prevents amyloidogenic processing of APP. Downregulation of SorLA has been found in patients with sporadic Alzheimer disease (AD). Currently SorLA has emerged as a potential target for AD therapy. (25404298)

Protocols

Product Specific Protocols
WB protocol for SORLA antibody 22592-1-APDownload protocol
IHC protocol for SORLA antibody 22592-1-APDownload protocol
IP protocol for SORLA antibody 22592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IP

Proc Natl Acad Sci U S A

α2A adrenergic receptor promotes amyloidogenesis through disrupting APP-SorLA interaction.

Authors - Yunjia Chen
mouseWB

Environ Res

Exposure to a real traffic environment impairs brain cognition in aged mice

Authors - Chenlu Xu
humanWB,IHC,IF,CoIP

Funct Integr Genomics

SORL1 stabilizes ABCB1 to promote cisplatin resistance in ovarian cancer

Authors - Zhen Zheng
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: mouse brain tissue