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SPAG9 Polyclonal antibody

SPAG9 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24423-1-AP

Synonyms

Cancer/testis antigen 89, CT89, HLC 6, HLC4, HSS, JIP 4, JLP, JNK interacting protein 4, KIAA0516, MAPK8IP4, PHET, PIG6, SPAG9, sperm associated antigen 9, Sperm specific protein, Sperm surface protein, Sunday driver 1, SYD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAG9 Polyclonal antibody (24423-1-AP)

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPAG9 Polyclonal antibody (24423-1-AP)

    WB analysis using 24423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SPAG9 Polyclonal antibody (24423-1-AP)

    WB analysis of NIH/3T3 using 24423-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG9 Polyclonal antibody (24423-1-AP)

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG9 Polyclonal antibody (24423-1-AP)

    IHC staining of human testis using 24423-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using SPAG9 Polyclonal antibody (24423-1-AP)

    IF Staining of RAW 264.7 using 24423-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 24423-1-AP (SPAG9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24423-1-AP targets SPAG9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG9 fusion protein Ag19763
Full Name sperm associated antigen 9
Calculated Molecular Weight 1321 aa, 146 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC146755
Gene Symbol SPAG9
Gene ID (NCBI) 9043
RRIDAB_2879541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPAG9 antibody 24423-1-APDownload protocol
IHC protocol for SPAG9 antibody 24423-1-APDownload protocol
IF protocol for SPAG9 antibody 24423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols