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  • Featured Product
  • KD/KO Validated

SPHK2 Polyclonal antibody

SPHK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17096-1-AP

Synonyms

SK 2, sphingosine kinase 2, SPHK2, SPK 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis of A549 using 17096-1-AP

    WB result of SPHK2 antibody (17096-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPHK2 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis of human kidney using 17096-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IP experiment of mouse liver using 17096-1-AP

    IP result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human breast cancer using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human breast cancer using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human kidney using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human kidney using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human liver using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human liver using 17096-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPHK2 Polyclonal antibody (17096-1-AP)

    IF Staining of HEK-293 using 17096-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPHK2 Polyclonal antibody (17096-1-AP)

    IF Staining of HeLa using 17096-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPHK2 antibody (17096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SPHK2 Polyclonal antibody (17096-1-AP)

    FC experiment of HeLa using 17096-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SPHK2 (17096-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inL02 cells, mouse brain tissue, human kidney tissue, A549 cells, mouse heart tissue, rat brain tissue, Raji cells, HCT 116 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17096-1-AP targets SPHK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPHK2 fusion protein Ag10569
Full Name sphingosine kinase 2
Calculated Molecular Weight 618 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010671
Gene Symbol SPHK2
Gene ID (NCBI) 56848
RRIDAB_10598479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).

Protocols

Product Specific Protocols
WB protocol for SPHK2 antibody 17096-1-APDownload protocol
IHC protocol for SPHK2 antibody 17096-1-APDownload protocol
IF protocol for SPHK2 antibody 17096-1-APDownload protocol
IP protocol for SPHK2 antibody 17096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Circ Res

Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension

Authors - A Dushani C U Ranasinghe
View Article
humanWB

Blood

Targeting sphingosine kinase 1 induces MCL1 dependent cell death in acute myeloid leukemia.

Authors - Jason A Powell
View Article
human,ratWB,IHC,IF

Bioact Mater

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Authors - Liwen Luo
View Article
humanWB,IF,IP

Oncogene

Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Authors - Heidi A Neubauer
View Article
humanWB

PLoS Pathog

Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells

Authors - Ingo Fohmann
View Article
humanCoIP

PLoS Pathog

Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection

Authors - Mengqiong Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yasuyo (Verified Customer) (02-23-2023)

Clear staining in mouse kidney paraffin section

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: kidney
SPHK2 Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:17096-1-AP)
FH

Hua (Verified Customer) (01-23-2023)

Good antibody. Works well on WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: mouse liver and mouse brain
SPHK2 Antibody Western Blot validation (1:4000 dilution) in mouse liver and mouse brain (Cat no:17096-1-AP)
FH

Jason (Verified Customer) (06-09-2021)

Clear staining in mouse liver paraffin section.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: liver
SPHK2 Antibody Immunohistochemistry validation (1:100 dilution) in liver (Cat no:17096-1-AP)
FH

David (Verified Customer) (05-04-2021)

It works for cell IF in vitro.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: A549 cell line
SPHK2 Antibody Immunofluorescence validation (1:100 dilution) in A549 cell line (Cat no:17096-1-AP)
FH

Chen (Verified Customer) (03-13-2021)

Works good for cell immunofluorescence assay in vitro.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Heart cells
SPHK2 Antibody Immunofluorescence validation ( dilution) in Heart cells (Cat no:17096-1-AP)
FH

Wei (Verified Customer) (03-12-2020)

Sharp and clear band from mouse heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart