STAT3 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, A431 cells, A549 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue, NIH/3T3 cells |
| Recommended dilution | WB: 1:1000-1:10000 |
IP = Immunoprecipitation
HeLa cells were subjected to SDS PAGE followed by western blot with 10253-2-AP(STAT3 Antibody) at dilution of 1:2000
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | STAT3 fusion protein ag0360 | Full name | signal transducer and activator of transcription 3 (acute-phase response factor) |
|
Calculated molecular weight |
770aa,88kd |
Observed molecular
weight |
88 kDa |
|
GenBank accession number |
BC000627 | Gene ID (NCBI) | 6774 |
| Gene symbol | STAT3 | Synonyms | APRF; FLJ20882; HIES; MGC16063 |
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. |
