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TBC1D22A Polyclonal antibody

TBC1D22A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18332-1-AP

Synonyms

C22orf4, HSC79E021, TBC1 domain family member 22A, TBC1 domain family, member 22A, TBC1D22A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of COLO 320 using 18332-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of HeLa using 18332-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    WB analysis of COLO 320 using 18332-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervical cancer using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human kidney using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human kidney using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervix using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using TBC1D22A Polyclonal antibody (18332-1-AP)

    IHC staining of human cervix using 18332-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D22A Polyclonal antibody (18332-1-AP)

    IF Staining of HeLa using 18332-1-AP

    Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells
Positive IHC detected inhuman cervical cancer tissue, human cervix tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18332-1-AP targets TBC1D22A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D22A fusion protein Ag12942
Full Name TBC1 domain family, member 22A
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC001292
Gene Symbol TBC1D22A
Gene ID (NCBI) 25771
RRIDAB_2302953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBC1D22A antibody 18332-1-APDownload protocol
IHC protocol for TBC1D22A antibody 18332-1-APDownload protocol
IF protocol for TBC1D22A antibody 18332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols