Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 18332-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18332-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 18332-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 18332-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18332-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervix using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervix using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 18332-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 18332-1-AP
Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TBC1D22A Polyclonal antibody

Name: TBC1D22A Polyclonal antibody
Brand: Proteintech
SKU: 18332-1-AP

TBC1D22A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18332-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TBC1 domain family, member 22A, TBC1 domain family member 22A, HSC79E021, C22orf4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, COLO 320 cells
Positive IHC detected in	human cervical cancer tissue, human cervix tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

18332-1-AP targets TBC1D22A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12942 Product name: Recombinant human TBC1D22A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-517 aa of BC001292 Sequence: MASDGARKQFWKRSNSKLPGSIQHVYGAQHPPFDPLLHGTLLRSTAKMPTTPVKAKRVSTFQEFESNTSDAWDAGEDDDELLAMAAESLNSKVVMETANRVLRNHSQRQGRPTLQEGPGLQQKPRPEAEPPSPPSGDLRLVKSVSESHTSCPAESASDAAPLQRSQSLPHSATVTLGGTSDPSTLSSSALSEREASRLDKFKQLLAGPNTDLEELRRLSWSGIPKPVRPMTWKLLSGYLPANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTEIFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSCVPAEVLCNIEADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFRWMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLFLQNLPTAHWDDEDISLLLAEAYRLKFAFADAPNHYKK Predict reactive species
Full Name	TBC1 domain family, member 22A
Calculated Molecular Weight	59 kDa
Observed Molecular Weight	59 kDa
GenBank Accession Number	BC001292
Gene Symbol	TBC1D22A
Gene ID (NCBI)	25771
RRID	AB_2302953
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WUA7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for TBC1D22A antibody 18332-1-AP	Download protocol
IHC protocol for TBC1D22A antibody 18332-1-AP	Download protocol
WB protocol for TBC1D22A antibody 18332-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Int J Med Sci High Expression of TBC1 Domain Family Member 22A is Related to Poor Prognosis in Ovarian Serous Cystadenocarcinoma Authors - Xiaofeng Lv View Article