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TFCP2 Polyclonal antibody
TFCP2 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15203-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells |
| Positive IP detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
15203-1-AP targets TFCP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TFCP2 fusion protein Ag7353 |
| Full Name | transcription factor CP2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC003634 |
| Gene Symbol | TFCP2 |
| Gene ID (NCBI) | 7024 |
| RRID | AB_2199582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| IP protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Pollut Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice. | ||
Cell Death Dis MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells. | ||
Clin Epigenetics DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter. | ||
Front Oncol TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer.
| ||
Exp Cell Res TFCP2, a binding protein of ATF3, promotes the progression of glioma by activating the synthesis of serine.
| ||
J Exp Clin Cancer Res Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis. |
