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- KD/KO Validated
Trefoil factor 1 Polyclonal antibody
Trefoil factor 1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13734-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human stomach tissue, human kidney tissue, mouse stomach tissue |
| Positive IHC detected in | human stomach tissue, mouse stomach tissue, human ovary tumor tissue, human stomach cancer tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
Product Information
13734-1-AP targets Trefoil factor 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Trefoil factor 1 fusion protein Ag4541 |
| Full Name | trefoil factor 1 |
| Calculated Molecular Weight | 84 aa, 9 kDa |
| Observed Molecular Weight | 9-12 kDa |
| GenBank Accession Number | BC032811 |
| Gene Symbol | TFF1 |
| Gene ID (NCBI) | 7031 |
| RRID | AB_2202853 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFF1 (formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Trefoil factor 1 antibody 13734-1-AP | Download protocol |
| IHC protocol for Trefoil factor 1 antibody 13734-1-AP | Download protocol |
| IF protocol for Trefoil factor 1 antibody 13734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
JCI Insight Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing. | ||
Bone Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.
| ||
Biomed Pharmacother Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.
| ||
Aesthet Surg J Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization. | ||
BMC Med Genomics Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection. |
