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KD/KO Validated

Talin-1 Polyclonal antibody

Talin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14168-1-AP

Publications(48)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ILWEQ, KIAA1027, talin 1, talin1, TLN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 750

Size/Concentration:

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14168-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 14168-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14168-1-AP (Talin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 14168-1-AP
IP result of anti-Talin-1 (IP:14168-1-AP, 4ug; Detection:14168-1-AP 1:40000) with HeLa cells lysate 1320 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14168-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 14168-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 14168-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14168-1-AP (Talin-1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HUVEC using 14168-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 14168-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 14168-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 14168-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 14168-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 14168-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14168-1-AP (Talin-1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HUVEC using 14168-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Talin-1 antibody (14168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 14168-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Polyclonal antibody (14168-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (48)
Protocols
Reviews (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HUVEC cells, HeLa cells, NIH/3T3 cells, C6 cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HUVEC cells, HeLa cells, HepG2 cells, A549 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14168-1-AP targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5367 Product name: Recombinant human TLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2193-2541 aa of BC042923 Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH Predict reactive species
Full Name	talin 1
Calculated Molecular Weight	2541 aa, 270 kDa
Observed Molecular Weight	250-270 kDa
GenBank Accession Number	BC042923
Gene Symbol	Talin-1
Gene ID (NCBI)	7094
RRID	AB_2271861
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y490
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
FC protocol for Talin-1 antibody 14168-1-AP	Download protocol
IF protocol for Talin-1 antibody 14168-1-AP	Download protocol
IHC protocol for Talin-1 antibody 14168-1-AP	Download protocol
IP protocol for Talin-1 antibody 14168-1-AP	Download protocol
WB protocol for Talin-1 antibody 14168-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	IF	ACS Nano Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. Authors - Xiao Huang View Article
human	WB,IP,IF	Proc Natl Acad Sci U S A Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4 Authors - Xiaochao Tan KD Validated View Article
human	WB	Antiviral Res MβCD inhibits SFTSV entry by disrupting lipid raft structure of the host cells Authors - Min Cheng View Article
human	WB,IP,IHC,IF	Elife Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer. Authors - Yixiao Zhang KD Validated View Article
human	IF	Mol Oncol ITIH5 as a multifaceted player in pancreatic cancer suppression, impairing tyrosine kinase signaling, cell adhesion and migration Authors - Jennifer Kosinski View Article
chicken	WB	Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. Authors - Beibei Zhang KD Validated View Article

Reviews

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3 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Hunter (Verified Customer) (01-30-2026)	Fantastic antibody, very clear and strong bands at a low dilution. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: Melanoma Cells
Udesh (Verified Customer) (08-12-2025)	Worked well for both WB and IF Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: WB 1:2000, IF, 1:300 Cell Tissue Type: MSCs
Elham (Verified Customer) (05-09-2025)	Really good staining. Used 1:200 dilution overnight at 4C and stained using Alexa Fluor 488 and actin Cell Mask 647 Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: Human Airway Smooth Muscle Cell Talin-1 Antibody Immunofluorescence validation (1:200 dilution) in Human Airway Smooth Muscle Cell (Cat no:14168-1-AP)