MKS3 Polyclonal antibody

MKS3 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13975-1-AP

Synonyms

JBTS6, Meckel syndrome type 3 protein, MECKELIN, MKS3, TMEM67, TNEM67, transmembrane protein 67



Tested Applications

Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13975-1-AP targets MKS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MKS3 fusion protein Ag5009
Full Name transmembrane protein 67
Calculated Molecular Weight 112 kDa
GenBank Accession NumberBC032835
Gene Symbol TMEM67
Gene ID (NCBI) 91147
RRIDAB_10638441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MKS3 antibody 13975-1-APDownload protocol
IF protocol for MKS3 antibody 13975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
mouseIF

Cell

The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.

Authors - Goetz Sarah C SC
humanWB,IF

Nat Cell Biol

CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.

Authors - Wang Won-Jing WJ
mouseIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
humanIF

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu