tubulin-beta Antibody
Rabbit Polyclonal
Catalog number: 10094-1-AP
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Applications
| Tested applications | ELISA, WB, IF, IP |
| Cited applications | WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, HepG2 cells, mouse brain tissue, PC-3 cells, SH-SY5Y cells |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of Tubulin Beta antibody (10094-1-AP for IP and Detection) with mouse brain tissue lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 10094-1-AP(tubulin-beta Antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using 10094-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | tubulin-beta fusion protein ag0136 | Full name | tubulin, beta 3 |
|
Calculated molecular weight |
50kd |
Observed molecular
weight |
55kd |
|
GenBank accession number |
BC000748 | Gene ID (NCBI) | 10381 |
| Gene symbol | TUBB3 | Synonyms | MC1R; TUBB4; beta-4 |
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
