TXN Antibody
Rabbit Polyclonal
Catalog number: 14999-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | CoIP, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Jurkat cells, A2780 cells, HeLa cells, human brain tissue, human liver tissue, mouse liver tissue |
| Positive IHC detected in | human liver |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 14999-1-AP(TXN Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human liver using 14999-1-AP(TXN Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver using 14999-1-AP(TXN Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TXN antibody 14999-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | TXN fusion protein ag6989 | Full name | thioredoxin |
|
Calculated molecular weight |
12kd |
Observed molecular
weight |
12kd |
|
GenBank accession number |
BC003377 | Gene ID (NCBI) | 7295 |
| Gene symbol | TXN | Synonyms | DKFZp686B1993; MGC61975; TRX; TRX1 |
Background
TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.This antibody is specific to TXN. |
