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TYW1B Polyclonal antibody

TYW1B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17665-1-AP

Synonyms

NCRNA00069, RSAFD2, TYW1B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using TYW1B Polyclonal antibody (17665-1-AP)

    WB analysis of mouse liver using 17665-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TYW1B Polyclonal antibody (17665-1-AP)

    WB analysis of MCF-7 using 17665-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using TYW1B Polyclonal antibody (17665-1-AP)

    IHC staining of human ovary using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TYW1B Polyclonal antibody (17665-1-AP)

    IHC staining of human ovary using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TYW1B Polyclonal antibody (17665-1-AP)

    IHC staining of human lung using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TYW1B Polyclonal antibody (17665-1-AP)

    IHC staining of human lung using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman ovary tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17665-1-AP targets TYW1B in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TYW1B fusion protein Ag11947
Full Name tRNA-yW synthesizing protein 1 homolog B (non-protein coding)
Calculated Molecular Weight 668 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC068520
Gene Symbol TYW1B
Gene ID (NCBI) 441250
RRIDAB_2211307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TYW1B antibody 17665-1-APDownload protocol
IHC protocol for TYW1B antibody 17665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols