UCP2 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | IF, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, Jurkat cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP(UCP2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP(UCP2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP(UCP2 Antibody) at Dilution 1:50 (under 40x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | UCP2 fusion protein ag1551 | Full name | uncoupling protein 2 (mitochondrial, proton carrier) |
|
Calculated molecular weight |
33kd |
Observed molecular
weight |
28-30kd |
|
GenBank accession number |
BC011737 | Gene ID (NCBI) | 7351 |
| Gene symbol | UCP2 | Synonyms | BMIQ4; SLC25A8; UCPH |
UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lyate be used for western blot assays. |
