VIM Antibody
Rabbit Polyclonal
Catalog number: 10366-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mosue, mouse, rat |
| Positive WB detected in | Jurkat cells, A549 cells, HeLa cells, MCF7 cells, NIH/3T3 cells, Raji cells |
| Positive IHC detected in | human lung cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 10366-1-AP(VIM Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human lung cancer using 10366-1-AP(VIM Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer using 10366-1-AP(VIM Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using VIM antibody 10366-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | VIM fusion protein ag0489 | Full name | vimentin |
|
Calculated molecular weight |
466aa,54kd |
Observed molecular
weight |
54kd |
|
GenBank accession number |
BC000163 | Gene ID (NCBI) | 7431 |
| Gene symbol | VIM | Synonyms | FLJ36605 |
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Mmonocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. |
