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VIM Antibody

Rabbit PolyClonal
Catalog No.: 10366-1-APpdfprint
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Applications
Tested Applications: ELISA,WB,IHC,IF
Specificity Reaction: Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology
Positive WB detected In:Jurkat cell,Hela cell;MCF7 cell;NIH3T3 cell;Raji cell
Positive IHC detected In:Lung cancer
Positive IF detected In:HepG2
Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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  • VIM western blot WB 10366-1-AP VIM Antibody VIM;FLJ36605 ;Jurkat cell were subjected to SDS PAGE followed by western blot with 10366-1-AP(VIM Antibody) at dilution of 1:2000
  • VIM IHC Immunohistochemical 10366-1-AP VIM Antibody VIM;FLJ36605 ;Immunohistochemistry of paraffin-embedded Lung cancer using 10366-1-AP(VIM Antibody) at Dilution 1:100 (under 10x lens)
  • VIM IHC Immunohistochemical 10366-1-AP VIM Antibody VIM;FLJ36605 ;Immunohistochemistry of paraffin-embedded Lung cancer using 10366-1-AP(VIM Antibody) at Dilution 1:100 (under 40x lens)
  • VIM Immunofluorescence IF 10366-1-AP VIM Antibody VIM;FLJ36605 ;Immunofluorescent analysis of HepG2 cells, using VIM antibody 10366-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
Source: Rabbit Purify: Antigen Affinity purified
IsoType: IgG Storage: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20oC, Avoid freeze / thaw cycles.
Immunogen Information
Immunogen: VIM fusion protein ag0489 Full Name: vimentin
Caculated Molecular
Weight: 		466aa,54kd Observed Molecular
Weight: 		54kd
GenbankNo BC000163 GeneID: 7431
Symbol VIM Synonyms VIM;FLJ36605 ;
Background

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Mmonocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes.

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