VPRBP Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, mouse testis tissue, PC-3 cells |
| Positive IHC detected in | human prostate cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
IP result of VPRBP antibody (11612-1-AP for IP and Detection) with HeLa cell lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP(VPRBP Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 11612-1-AP(VPRBP Antibody) at Dilution 1:50 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | VPRBP fusion protein ag2184 | Full name | Vpr (HIV-1) binding protein |
|
Calculated molecular weight |
1506aa,169kd |
Observed molecular
weight |
169kd |
|
GenBank accession number |
BC022792 | Gene ID (NCBI) | 9730 |
| Gene symbol | VPRBP | Synonyms | DCAF1; KIAA0800; MGC102804 |
VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063). |
