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Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 26520-1-AP
Publications(256)
Reviews (14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

CMD1W, Metavinculin, MV, MVCL, VCL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
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  • Western Blot (WB) analysis of various lysates using Vinculin Polyclonal antibody (26520-1-AP)

    WB analysis using 26520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of mouse skeletal muscle using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of mouse skeletal muscle using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse colon tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of mouse colon using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse colon tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of mouse colon using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human skeletal muscle using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human skeletal muscle using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human breast cancer using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human breast cancer using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human prostate cancer using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IHC staining of human prostate cancer using 26520-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of mouse colon using 26520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (26520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of mouse colon using 26520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (26520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of A549 using 26520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Vinculin antibody (26520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of A549 using 26520-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Vinculin antibody (26520-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of HepG2 using 26520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Vinculin antibody (26520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vinculin Polyclonal antibody (26520-1-AP)

    IF Staining of HepG2 using 26520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Vinculin antibody (26520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Flow cytometry (FC) experiment of HepG2 cells using Vinculin Polyclonal antibody (26520-1-AP)

    FC experiment of HepG2 using 26520-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inHepG2 cells, A549 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:60000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26520-1-AP targets Vinculin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Predict reactive species
Full Name vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_2868558
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
FC protocol for Vinculin antibody 26520-1-APDownload protocol
IF protocol for Vinculin antibody 26520-1-APDownload protocol
IHC protocol for Vinculin antibody 26520-1-APDownload protocol
WB protocol for Vinculin antibody 26520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

SpeciesApplicationTitle
humanWB,IF

Cell Metab

Electron transport chain inhibition increases cellular dependence on purine transport and salvage

Authors - Zheng Wu
View Article
humanWB

Blood

Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia

Authors - Xinyue Zhou
View Article
humanWB,IF

ACS Nano

Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release

Authors - Lu Zhang
View Article
humanWB

Mol Cell

Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs

Authors - Yuanfan Sun
View Article
mouseIF

Adv Sci (Weinh)

Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.

Authors - Mu Li
View Article
rabbitWB,IF

Adv Sci (Weinh)

Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing

Authors - Yingni Xu
View Article

Reviews

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14 verified customer reviews

What customers say

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Audrey (Verified Customer) (04-10-2026)

Works well for Western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/5000

Xiaoping (Verified Customer) (03-03-2026)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BxPC3

Ryan (Verified Customer) (02-04-2026)

Great antibody at good price.

  • Applications: Western Blot
  • Cell Tissue Type: 3t3-L1

Nancy (Verified Customer) (01-30-2026)

Very good antibody. I always get consistent results when I use it for WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fibroblast cell line
Vinculin Antibody Western Blot validation (1:2000 dilution) in Fibroblast cell line (Cat no:26520-1-AP)

Monica (Verified Customer) (11-14-2025)

Great antibody for Vinculin. I wanted a higher molecular weight loading control antibody. This worked so well. 1.5hr at RT for primary at secondary for 1 hr. Gave beautiful clean band. Very happy with this.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:40,000
  • Cell Tissue Type: Glomerular endothelail cells
Vinculin Antibody Western Blot validation (1:40,000 dilution) in Glomerular endothelail cells (Cat no:26520-1-AP)

JIN-FENG (Verified Customer) (10-08-2025)

The vinculin antibody works very well and very specific on my WB analysis.

  • Applications: Western Blot, Cell culture
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: U2OS cell line
Vinculin Antibody Western Blot,Cell culture validation (1:5000 dilution) in U2OS cell line (Cat no:26520-1-AP)

Tsimafei (Verified Customer) (09-22-2025)

Excellent and specific antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: U2OS
Vinculin Antibody Western Blot validation (1:10000 dilution) in U2OS (Cat no:26520-1-AP)

Elham (Verified Customer) (06-18-2025)

Human airway smooth muscle cells fixed after 1 h of plating. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X 100. Blocked with 0.1% BSA and incubated with primary antibody overnight at 4C at 1:100 dilution. Following day cells were washed and incubated for 1 h with Alexa 594 at 1:400 dilution.

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:100
Vinculin Antibody Immunofluorescence,Cell culture validation (1:100 dilution) in (Cat no:26520-1-AP)

Siavash (Verified Customer) (01-17-2025)

The antibody was used for immunofluorescence staining of 2D cultured cells fixed with 1%PFA and permeabilized with Triton X100. Cells were incubated overnight at +4 degrees celcius. The staining was visible in both epifluorescence microscope and confocal fluorescence microscope. Also, used for immunostaining of formalin-fixed paraffin-embedded (FFPE) skin of mouse. The antigen retrieval was citrate buffer. Primary antibody was incubated overnight at +4 degrees celcius.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: Cell: 1:300, Tissue: 1:100
  • Cell Tissue Type: Human Primary Epidermal Keratinocytes, Mouse Skin
Vinculin Antibody Immunofluorescence validation (Cell: 1:300, Tissue: 1:100 dilution) in Human Primary Epidermal Keratinocytes, Mouse Skin (Cat no:26520-1-AP)

Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HEKs / HUVEC
Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 in TBST 5% Milk
  • Cell Tissue Type: U2OS
Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse pancreatic cancer cell line
Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Epithelial cell
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