WARS Antibody
Rabbit Polyclonal
Catalog number: 16081-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human ovary tumor |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HepG2 cells were subjected to SDS PAGE followed by western blot with 16081-1-AP(WARS Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human ovary tumor using 16081-1-AP(WARS Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor using 16081-1-AP(WARS Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | WARS fusion protein ag9079 | Full name | tryptophanyl-tRNA synthetase |
|
Calculated molecular weight |
471aa,53kd |
Observed molecular
weight |
53kd |
|
GenBank accession number |
BC017489 | Gene ID (NCBI) | 7453 |
| Gene symbol | WARS | Synonyms | GAMMA-2; IFI53; IFP53 |
Background
WARS is also named as IFI53, WRS,TrpRS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing. This antibody is specific to WARS. |
