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ZCRB1 Polyclonal antibody

ZCRB1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25629-1-AP

Synonyms

MADP 1, MADP1, RBM36, U11/U12 31K, U11/U12 snRNP 31 kDa protein, ZCCHC19, ZCRB1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of mouse testis using 25629-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of SMMC-7721 using 25629-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of Neuro-2a using 25629-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of Raji using 25629-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of Raji using 25629-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis of mouse testis using 25629-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZCRB1 Polyclonal antibody (25629-1-AP)

    IF Staining of HepG2 using 25629-1-AP

    Immunofluorescent analysis of HepG2 cells using 25629-1-AP (ZCRB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, Raji cells, Neuro-2a cells, SMMC-7721 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25629-1-AP is ZCRB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZCRB1 fusion protein Ag22095
Full Name zinc finger CCHC-type and RNA binding motif 1
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC022543
Gene Symbol ZCRB1
Gene ID (NCBI) 85437
RRIDAB_2880169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZCRB1 antibody 25629-1-APDownload protocol
IF protocol for ZCRB1 antibody 25629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.

Authors - Jian Song
View Article