b-cat Antibody
Rabbit Polyclonal
Catalog number: 51067-2-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat, pig; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells, human placenta tissue, MCF7 cells, mouse liver tissue, mouse lung tissue, NIH/3T3 cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-CTNNB1 (51067-2-AP for IP and Detection) with 293 cell lysate.
HeLa cells were subjected to SDS PAGE followed by western blot with 51067-2-AP(b-cat Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer using 51067-2-AP(b-cat Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer using 51067-2-AP(b-cat Antibody) at Dilution 1:100 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | catenin (cadherin-associated protein), beta 1, 88kDa |
|
Calculated molecular weight |
781aa,86kd |
Observed molecular
weight |
90kd |
|
GenBank accession number |
BC058926 | Gene ID (NCBI) | 1499 |
| Gene symbol | CTNNB1 | Synonyms | CTNNB; DKFZp686D02253; FLJ25606; FLJ37923 |
Background
β-catenin is an evolutionarily conserved, multifunctional intracellular protein. β-catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to α-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) |
