mCherry Polyclonal antibody

mCherry Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (6)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26765-1-AP

Synonyms

mCherry



Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26765-1-AP is mCherry Fusion Protein expressed in E. coli.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, D. punctatus, yeast, zebrafish, plant
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen mCherry fusion protein Ag25320
Full Name mCherry
Calculated Molecular Weight 27 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2876881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.

Protocols

Product Specific Protocols
WB protocol for mCherry antibody 26765-1-APDownload protocol
IF protocol for mCherry antibody 26765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Extracell Vesicles

Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion

Authors - Chuqi Liu
humanIP

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
humanIP

Protein Cell

cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.

Authors - Xiaocui Li
human IF,IP

Dev Cell

ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress

Authors - Di Chen
IF

Dev Cell

Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.

Authors - Guobin Xie
WB,IF

Autophagy

Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation.

Authors - Xiaoxiang Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Verdiana (Verified Customer) (10-12-2023)

Nice and clear bands!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
FH

Andrea (Verified Customer) (10-05-2023)

Good and strong signal for the mCherry fusion protein. In my case it was a 1:1000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT-116
mCherry Antibody Western Blot validation (1:1000 dilution) in HCT-116 (Cat no:26765-1-AP)
FH

Tatyana (Verified Customer) (01-09-2023)

Excellent antibody, very good bright signal, no background or non-specific bands. HeLa cells were transfected with plasmids for expression of mCherry tagged proteins, lysed after 24 hours, followed by WB (semi-dry transfer, blocking in 4% milk). Antibody was diluted in 4% milk.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human / HeLa cells
mCherry Antibody Western Blot validation (1:2000 dilution) in Human / HeLa cells (Cat no:26765-1-AP)
FH

Laura (Verified Customer) (01-14-2020)

Antibody was used in 5% TBST overnight, gave a clean blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T
FH

Benjamin (Verified Customer) (12-10-2019)

This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T Lysate
FH

Liang (Verified Customer) (05-27-2019)

The antibody works perfect. According the attached image, The 2nd from the left is the negative control protein (mGFP) and the 3rd lane is the mCherry protein as the positive control, the others are the target protein.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: purified protein expressed from Ecoli
mCherry Antibody Western Blot,Immunoprecipitation, validation (1:3000 dilution) in purified protein expressed from Ecoli (Cat no:26765-1-AP)