Tubulin-Alpha Antibody
Mouse Monoclonal
Catalog number: 66031-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive IHC detected in | human liver cancer |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody 66031-1-Ig at a dilution of 1:10000.
Western blot of Hela cell with anti-Tubulin-Alpha (66031-1-Ig) at various dilutions.
Immunohistochemistry of paraffin-embedded human liver cancer using 66031-1-Ig(Tubulin-Alpha Antibody) at Dilution 1:100 (under 40x lens)
Immunohistochemistry of paraffin-embedded human liver cancer using 66031-1-Ig(Tubulin-Alpha Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using TUBA1B antibody 66031-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Tubulin-Alpha fusion protein ag18034 | Full name | tubulin, alpha 1b |
|
Calculated molecular weight |
50kd |
Observed molecular
weight |
52kD |
|
GenBank accession number |
BC009314 | Gene ID (NCBI) | 10376 |
| Gene symbol | TUBA1B | Synonyms | K-ALPHA-1 |
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antibody recognize Tubulin Alpha only. |
