ubiquitin Antibody
Rabbit Polyclonal
Catalog number: 10201-2-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:10-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 10201-2-AP(ubiquitin Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human ovary tumor using 10201-2-AP(ubiquitin Antibody) at Dilution 1:10 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | ubiquitin fusion protein ag0260 | Full name | ubiquitin B |
|
Calculated molecular weight |
26kd |
Observed molecular
weight |
16 kDa, 24 kDa |
|
GenBank accession number |
BC000379 | Gene ID (NCBI) | 7314 |
| Gene symbol | UBB | Synonyms | FLJ25987; MGC8385 |
Background
Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. |
