ARPP-21 Polyclonal antibody

ARPP-21 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11829-1-AP

Synonyms

ARPP 21, ARPP21, ARPP-21, RCS, TARPP



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11829-1-AP targets ARPP-21 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPP-21 fusion protein Ag2399
Full Name cyclic AMP-regulated phosphoprotein, 21 kD
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC017805
Gene Symbol ARPP-21
Gene ID (NCBI) 10777
RRIDAB_2877798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARPP-21 antibody 11829-1-APDownload protocol
IHC protocol for ARPP-21 antibody 11829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan
mouseWB

Nat Commun

The thymocyte-specific RNA-binding protein Arpp21 provides TCR repertoire diversity by binding to the 3'-UTR and promoting Rag1 mRNA expression

Authors - Meng Xu