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CoraLite® Plus 750 Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

MPC-11

Cat no : CL750-65128

Synonyms

gamma2b, IgG2b, IgG2b Isotype Control, Igh-3, IGHG2B, Mouse IgG2b Isotype Control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Mouse IgG2b Isotype Control (MP (CL750-65128)

    FC experiment of human PBMCs using CL750-65128

    1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 750 Mouse IgG2b Isotype Control (CL750-65128, Clone:MPC-11) (red), Anti-Human CD20 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name immunoglobulin heavy chain 3 (serum IgG2b)
Gene Symbol IgG2b Isotype Control
Gene ID (NCBI) 16016
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 IgG2b Isotype Control antibody CL750-65128Download protocol
Standard Protocols
Click here to view our Standard Protocols