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ATF2-Specific Polyclonal antibody

ATF2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14834-1-AP

Synonyms

ATF2, ATF2-Specific, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, C2C12 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14834-1-AP targets ATF2-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name activating transcription factor 2
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC026175
Gene Symbol ATF2
Gene ID (NCBI) 1386
RRIDAB_10694139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ).

Protocols

Product Specific Protocols
WB protocol for ATF2-Specific antibody 14834-1-APDownload protocol
IHC protocol for ATF2-Specific antibody 14834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB

BMC Med

Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

Authors - Zhijian Zheng
mouseWB

Cell Rep

Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.

Authors - Lu Yao
humanWB

J Biol Chem

MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.

Authors - Fanpeng Zhao
humanWB

Artif Cells Nanomed Biotechnol

Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.

Authors - Nan Li
  • KD Validated