B23/NPM1 Recombinant antibody, PBS Only

B23/NPM1 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4

Cat no : 82030-1-PBS

Synonyms

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Validation Data Gallery

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  WB analysis using 82030-1-RR (same clone as 82030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82030-1-RR (B23 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 82030-1-RR (same clone as 82030-1-PBS)

  IP result of anti-B23/NPM1 (IP:82030-1-RR, 4ug; Detection:82030-1-RR 1:8000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82030-1-RR (same clone as 82030-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23 antibody (82030-1-RR, Clone: 1G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82030-1-PBS targets B23/NPM1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: B23/NPM1 fusion protein Ag0286
• Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC002398
• Gene Symbol: NPM1
• Gene ID (NCBI): 4869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.