Bcl-XL Recombinant antibody

Bcl-XL Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8

Cat no : 83330-1-RR

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092

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Validation Data Gallery

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  WB analysis using 83330-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83330-1-RR (Bcl-xL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83330-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (83330-1-RR, Clone: 1E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83330-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-XL Recombinant antibody (83330-1-RR, Clone:1E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Ramos cells, K-562 cells, RAW 264.7 cells, C6 cells, Jurkat cells
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:125-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83330-1-RR is Bcl-XL Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag25604
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.