CD206 Recombinant antibody, PBS Only

CD206 Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, Mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G15

Cat no : 81525-1-PBS

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Validation Data Gallery

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  WB analysis using 81525-1-RR (same clone as 81525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81525-1-PBS targets CD206 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse samples.

• Tested Reactivity: Human, Pig, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: MRC1
• Gene ID (NCBI): 4360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.