CoraLite®488-conjugated CD22 Monoclonal antibody

CD22 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

7A2F1

Cat no : CL488-66103

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66103

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66103 (CD22 antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-66103

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66103 (CD22 antibody) at dilution of 1:100.

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  IF Staining of human appendicitis using CL488-66103

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®488 CD22 antibody (CL488-66103, Clone: 7A2F1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66103 is CD22 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD22 fusion protein Ag17986
• Full Name: CD22 molecule
• Calculated Molecular Weight: 847 aa, 95 kDa
• GenBank Accession Number: BC109306
• Gene Symbol: CD22
• Gene ID (NCBI): 933
• RRID: AB_2883259
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 491 nm / 516 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.