CD27 Monoclonal antibody, PBS Only

CD27 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1G3

Cat no : 66308-1-PBS

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

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Validation Data Gallery

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  WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66308-1-Ig (same clone as 66308-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Raji using 66308-1-Ig (same clone as 66308-1-PBS)

  1X10^6 Raji cells were surface stained with 0.2 ug Anti-Human CD27 (66308-1-Ig, Clone:1C1G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were not fixed. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66308-1-PBS targets CD27 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD27 fusion protein Ag24537
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.