CD79A Polyclonal antibody

CD79A Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22349-1-AP

Synonyms

CD79A, Ig alpha, IGA, MB 1, MB 1 membrane glycoprotein, MB1, Surface IgM associated protein

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Validation Data Gallery

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  WB analysis using 22349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22349-1-AP (CD79A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 22349-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22349-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 22349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 22349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 22349-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79A antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 22349-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79A antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Raji using 22349-1-AP

  1X10^6 Raji cells were stained with 0.20 ug Anti-Human CD79A (22349-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22349-1-AP is CD79A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD79A fusion protein Ag17924
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• Calculated Molecular Weight: 226 aa, 25 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC113733
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• RRID: AB_2879084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD79A, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.