CHRNB1 Polyclonal antibody

CHRNB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11553-1-AP

Synonyms

ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS



Tested Applications

Positive WB detected inhuman liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

11553-1-AP targets CHRNB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNB1 fusion protein Ag2108
Full Name cholinergic receptor, nicotinic, beta 1 (muscle)
Calculated Molecular Weight 501 aa, 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC023553
Gene Symbol CHRNB1
Gene ID (NCBI) 1140
RRIDAB_2291854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits. CHRNB1 gene encodes acetylcholine receptor subunit beta (ACHRB). Mutations in this gene are associated with slow-channel congenital myasthenic syndrome.

Protocols

Product Specific Protocols
WB protocol for CHRNB1 antibody 11553-1-APDownload protocol
IHC protocol for CHRNB1 antibody 11553-1-APDownload protocol
IP protocol for CHRNB1 antibody 11553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

Genes Immun

Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of na?ve CD4 T cells toward the Th1, Th2 and Th17 lineages.

Authors - Qian J J
mouseWB

Biochem Biophys Res Commun

Association of a newly identified lncRNA LNC_000280 with the formation of acetylcholine receptor clusters in vitro.

Authors - Jin Deng
mouseWB

Mol Ther Nucleic Acids

MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury

Authors - Zhidan Qi