• Featured Product
  • KD/KO Validated

CRISP3 Polyclonal antibody

CRISP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14847-1-AP

Synonyms

Aeg2, CRISP 3, CRISP3, CRS3, SGP28



Tested Applications

Positive WB detected inhuman saliva, human placenta tissue, Jurkat cells, mouse colon tissue, mouse ovary tissue, mouse thymus tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14847-1-AP targets CRISP3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cysteine-rich secretory protein 3
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberNM_006061
Gene Symbol CRISP3
Gene ID (NCBI) 10321
RRIDAB_1607510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Protocols

Product Specific Protocols
WB protocol for CRISP3 antibody 14847-1-APDownload protocol
IHC protocol for CRISP3 antibody 14847-1-APDownload protocol
IP protocol for CRISP3 antibody 14847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Natl Cancer Inst

Progesterone Receptor Isoform Ratio: A Breast Cancer Prognostic and Predictive Factor for Antiprogestin Responsiveness.

Authors - Paola A Rojas
humanIHC

J Hematol Oncol

Cysteine- rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis.

Authors - Samir Al Bashir
humanWB,IHC

Int J Biol Sci

Circular RNA hsa_circ_0003823 promotes the Tumor Progression, Metastasis and Apatinib Resistance of Esophageal Squamous Cell Carcinoma by miR-607/CRISP3 Axis

Authors - Yu-Ming Wang
  • KD Validated
humanWB

J Dent Res

Proteome Analysis of Temporomandibular Joint with Disc Displacement

Authors - X Liu