CSF1R Polyclonal antibody
CSF1R Polyclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 25949-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue, human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 6 publications below |
| IF | See 1 publications below |
Product Information
25949-1-AP targets CSF1R in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CSF1R fusion protein Ag22845 |
| Full Name | colony stimulating factor 1 receptor |
| Calculated Molecular Weight | 972 aa, 108 kDa |
| GenBank Accession Number | BC047521 |
| Gene Symbol | CSF1R |
| Gene ID (NCBI) | 1436 |
| RRID | AB_2880306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CSF1R antibody 25949-1-AP | Download protocol |
| IF protocol for CSF1R antibody 25949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies. | ||
CNS Neurosci Ther A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma | ||
Front Genet Human placenta-based genome-wide mRNA sequencing to identify TEK/IGF1/CSF1/ANGPT2 as crucial segments in the pathogenesis of pre-eclampsia | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues | ||
Int J Exp Pathol Inhibition of inflammation and infiltration of M2 macrophages in NSCLC through the ATF3/CSF1 axis: Role of miR-27a-3p |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jaouhara (Verified Customer) (12-05-2023) | Used in synovium PFA-fixed slides to detect macrophages. Works well.
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