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  • KD/KO Validated

CUL2 Polyclonal antibody

CUL2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10981-2-AP

Synonyms

CUL 2, CUL2, cullin 2



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10981-2-AP targets CUL2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL2 fusion protein Ag1434
Full Name cullin 2
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC009591
Gene Symbol CUL2
Gene ID (NCBI) 8453
RRIDAB_2086422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL2 antibody 10981-2-APDownload protocol
IHC protocol for CUL2 antibody 10981-2-APDownload protocol
IP protocol for CUL2 antibody 10981-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Authors - Manuela Antonioli
  • KD Validated
humanWB

Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang
humanWB

Oncogene

iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.

Authors - Dong Zhao
humanWB

Aging (Albany NY)

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.

Authors - Xiao Dong
  • KD Validated
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
  • KD Validated
humanWB

J Cell Sci

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Authors - Tomoaki Nagai
  • KD Validated