HDAC2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HDAC2 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3E4

Cat no : 67165-1-PBS

Synonyms

HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1

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Validation Data Gallery

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  WB analysis using 67165-1-Ig (same clone as 67165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67165-1-Ig (same clone as 67165-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67165-1-Ig (HDAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67165-1-Ig (same clone as 67165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67165-1-Ig (same clone as 67165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67165-1-Ig (HDAC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67165-1-Ig (same clone as 67165-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC2 antibody (67165-1-Ig, Clone: 1A3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67165-1-Ig (same clone as 67165-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HDAC2 (67165-1-Ig, Clone:1A3E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67165-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67165-1-PBS targets HDAC2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HDAC2 fusion protein Ag21288
• Full Name: histone deacetylase 2
• Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa,55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC031055
• Gene Symbol: HDAC2
• Gene ID (NCBI): 3066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.