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ICOSLG Monoclonal antibody, PBS Only

ICOSLG Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1F8

Cat no : 67003-1-PBS

Synonyms

B7 H2, B7 homolog 2, B7 like protein Gl50, B7 related protein 1, B7H2, B7RP 1, B7RP1, CD275, GL50, ICOS L, ICOS ligand, ICOSL, ICOSLG, KIAA0653, LICOS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICOSLG Monoclonal antibody (67003-1-Ig)

    WB analysis using 67003-1-Ig (same clone as 67003-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67003-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using ICOSLG Monoclonal antibody (67003-1-Ig)

    WB analysis of Daudi using 67003-1-Ig (same clone as 67003-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67003-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67003-1-PBS targets ICOSLG in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ICOSLG fusion protein Ag6799
Full Name inducible T-cell co-stimulator ligand
Calculated Molecular Weight 33 kDa, 34 kDa
Observed Molecular Weight 60-65 kDa, 28-35 kDa
GenBank Accession NumberBC064637
Gene Symbol ICOSLG
Gene ID (NCBI) 23308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).