IHCeasy CX3CL1 Ready-To-Use IHC Kit
CX3CL1 Ready-to-use reagent kit for IHC.
Cat no : KHC1070
Synonyms
ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1
Validation Data Gallery
Product Information
KHC1070 is a ready-to-use IHC kit for staining of CX3CL1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy CX3CL1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75 kDa precursor and undergoes glycosylation to yield two mature forms: a 100 kDa cell membrane bound form and an 85 kDa soluble form which is released from the cell surface. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1 |
