IL3RA/CD123 Polyclonal antibody

IL3RA/CD123 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13655-1-AP

Synonyms

CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha, IL 3R subunit alpha, IL 3RA, IL3R, IL3RA, IL-3RA, IL3RAY, IL3RX, IL3RY

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Validation Data Gallery

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  WB analysis of HL-60 using 13655-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13655-1-AP (IL3RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13655-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13655-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13655-1-AP (IL3RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13655-1-AP targets IL3RA/CD123 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL3RA/CD123 fusion protein Ag4452
• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: IL3RA
• Gene ID (NCBI): 3563
• RRID: AB_10598015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.