MultiProTM 5CFLX Anti-Human SP1 (4G3H3)

SP1 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

4G3H3

Cat no : G66508-1-5C

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1



Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66508-1-5C targets SP1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen SP1 fusion protein Ag16720
Full Name MultiProTM 5CFLX Anti-Human SP1 (4G3H3)
Calculated Molecular Weight 785 aa, 81 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
Conjugate 5CFLX
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol