NOTCH2 Polyclonal antibody
NOTCH2 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 28580-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-87 MG cells |
Positive IP detected in | HEK-293 cells, U-87 MG cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Product Information
28580-1-AP targets NOTCH2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NOTCH2 fusion protein Ag29413 |
Full Name | Notch homolog 2 (Drosophila) |
Calculated Molecular Weight | 265 kDa |
Observed Molecular Weight | 110 kDa, 265 kDa |
GenBank Accession Number | NM_024408 |
Gene Symbol | NOTCH2 |
Gene ID (NCBI) | 4853 |
RRID | AB_2881175 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for NOTCH2 antibody 28580-1-AP | Download protocol |
IP protocol for NOTCH2 antibody 28580-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer. | ||
Life Sci GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma. | ||
Ann Transl Med SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway. | ||
Transl Androl Urol Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer. | ||
Front Oncol A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer |