CoraLite®594-conjugated Neuropilin 1 Monoclonal antibody
Neuropilin 1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2H3F6
Cat no : CL594-60067
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-60067 targets Neuropilin 1 in FC (Intra) applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Neuropilin 1 fusion protein Ag0931 |
Full Name | neuropilin 1 |
Calculated Molecular Weight | 103 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC007737 |
Gene Symbol | NRP1 |
Gene ID (NCBI) | 8829 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
Protocols
Product Specific Protocols | |
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FC protocol for CL594 Neuropilin 1 antibody CL594-60067 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |