NeutraKine® IL-6 Monoclonal antibody, PBS Only

NeutraKine® IL-6 Monoclonal Antibody for FC, IHC, ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC, IHC, ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

4G3E6

Cat no : 69001-1-PBS

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6, NeutraKine® IL-6

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

  Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL. This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

• 

  Neutralization experiment of NeutraKine® IL-6 using 69001-1-Ig (same clone as 69001-1-PBS)

  Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 3-15 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69001-1-Ig (same clone as 69001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-1080 using 69001-1-Ig (same clone as 69001-1-PBS)

  1X10^6 HT-1080 cells treated with human IL17F (HZ-1116) for 24 hours in the presence of protein transport inhibitor were treated with fixation and permeabilization reagents and intracellularly stained with 0.5 ug Anti-Human NeutraKine® IL-6 (69001-1-Ig, Clone:4G3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. This data was developed using the same antibody clone with 69001-1-PBS in a different storage buffer formulation.

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Product Information

69001-1-PBS targets NeutraKine® IL-6 in FC, IHC, ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-6 protein HZ-1019
• Full Name: interleukin 6 (interferon, beta 2)
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody can be used to neutralize the bioactivity of IL-6.