• Featured Product
  • KD/KO Validated

PDCD6 Polyclonal antibody

PDCD6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12303-1-AP

Synonyms

ALG 2, ALG2, PDCD6, PEF1B, programmed cell death 6



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12303-1-AP targets PDCD6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD6 fusion protein Ag2949
Full Name programmed cell death 6
Calculated Molecular Weight 191 aa, 22 kDa
Observed Molecular Weight 15-22 kDa
GenBank Accession NumberBC012384
Gene Symbol PDCD6
Gene ID (NCBI) 10016
RRIDAB_2162459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDCD6 antibody 12303-1-APDownload protocol
IHC protocol for PDCD6 antibody 12303-1-APDownload protocol
IP protocol for PDCD6 antibody 12303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Structural basis for dimerization quality control.

Authors - Elijah L Mena
humanIF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra
humanWB,IF

Cell

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Authors - Colleen A McGourty
  • KD Validated
humanWB

Mol Cell

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Authors - David Akopian
humanWB,IF

Cell Rep

Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.

Authors - Johannes Westman
  • KD Validated
humanIF

J Cell Biol

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters

Authors - Femmy C Stempels