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  • KD/KO Validated

PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12952-1-AP

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18



Tested Applications

Positive WB detected inmouse lung tissue, MCF-7 cells, A549 cells
Positive IHC detected inmouse testis tissue, human lung cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12952-1-AP targets PLK4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK4 fusion protein Ag3605
Full Name polo-like kinase 4 (Drosophila)
Calculated Molecular Weight 970 aa, 109 kDa
Observed Molecular Weight 105-112 kDa
GenBank Accession NumberBC036023
Gene Symbol PLK4
Gene ID (NCBI) 10733
RRIDAB_2284150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PLK4 antibody 12952-1-APDownload protocol
IHC protocol for PLK4 antibody 12952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse, humanWB, IHC

Nat Commun

Loss of KLF14 triggers centrosome amplification and tumorigenesis.

Authors - Guangjian Fan
  • KO Validated
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie
mouseIF

EMBO Rep

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Authors - Ting Song
humanWB,IHC

Cell Death Dis

Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.

Authors - Xiangdong Tian
  • KD Validated
humanWB

Cell Death Dis

NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.

Authors - Min Li
humanWB

Mol Cancer Ther

Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.

Authors - Ke Liu