PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22163-1-AP

Synonyms

PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22163-1-AP targets PXMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PXMP3 fusion protein Ag17815
Full Name peroxisomal membrane protein 3, 35kDa
Calculated Molecular Weight 305 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000661
Gene Symbol PXMP3
Gene ID (NCBI) 5828
RRIDAB_2879014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PXMP3 antibody 22163-1-APDownload protocol
IHC protocol for PXMP3 antibody 22163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses.

Authors - Anu S Nath